Contents

xv

16.4.1

Nutrients Availability

248

16.4.2

Hydraulic Retention Time (HRT) and Solid Retention Time (SRT)

250

16.4.3

Design of Biohydrogen-Producing Reactor

250

16.4.4

Substrate Concentration

250

16.4.5

Temperature and pH

251

16.4.6

Seed Culture

251

16.4.7

Hydrogen Partial Pressure

251

16.5

Techniques to Improve Biohydrogen Production

252

16.5.1

Reactor Design and Configuration

252

16.5.2

Microbial Consortia

252

16.6

Environmental and Economic Assessment of BioHydrogen Production

in AnMBRs

253

16.7

Future Perspectives of Biohydrogen Production

253

16.8

Products Based on Solid-State Fermenter

253

16.8.1

Bioactive Products

253

16.8.2

Enzymes

254

16.8.3

Organic Acids

255

16.8.4

Biopesticides

256

16.8.5

Aroma Compounds

256

16.8.6

Bio-Pigment Production

257

16.8.7

Miscellaneous Compounds

257

16.9

Koji Fermenters for SSF for Production of Different Chemicals

257

16.10

Recent Research on Biofuel Manufacturing in Bioreactors Other than

Biohydrogen

258

References

259

Part VI

Waste2Energy with Biotechnology: Feasibilities and

Challenges

263

17

Utilization of Microbial Potential for Bioethanol Production

from Lignocellulosic Waste

265

Manisha Rout, Bithika Sardar, Puneet K. Singh, Ritesh Pattnaik, and

Snehasish Mishra

17.1

Introduction

265

17.1.1

Bioethanol from Different Feed Stocks

265

17.1.2

Sources of Lignocellulosic Biomass

266

17.1.3

Structure and Composition of Lignocellulose

266

17.1.4

Challenges in Bioethanol Production from LCB

267

17.2

Processing of Lignocellulosic Biomass to Ethanol

268

17.3

Biological Pretreatment

271

17.3.1

Potential Microorganisms Involved in Lignin Degradation

272

17.3.1.1

Lignin Degrading Fungi

272

17.3.1.2

Lignin-Degrading Bacteria

274

17.3.2

Mechanism Involved in Delignification

274